Overview

MyoMETH showcases the results of the paper: Integrated fibre-specific methylome and proteome profiling of human skeletal muscle across males and females with fibre-type deconvolution. We have utilised DNA methylation results with linked protein abundance measures to create a data repository (MyoMETH) for further exploration. We have also generated a fibre-type DNAm reference matrix of TI and TII human muscle fibres and leveraged deconvolution algorithms to estimate fibre-type proportions of muscle samples from whole skeletal muscle DNAm data that we provide as a web tool (MyoTYPE).

Approach

We isolated >200 fibres per sample from 7 males and 5 females and typed them as Type I slow and Type II fast muscle fibres. We simultaneously extracted proteins and DNA from pooled TI and TII fibres for all 12 samples. We subsequently assessed protein abundance by liquid chromatography tandem mass spectrometry and DNA methylation using the new EPICv2 Illumina Infinium array. We assessed DNAm from 15 whole muscle samples for interrogation of fibre-type influence on whole muscle DNAm patterns (we had matching WM DNAm data for our 12 pooled fibre-type samples). For full details please refer to the paper citation below.

Citation

If you use MyoMETH in your research, please consider citing:

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